We use an assortment of fluorescently tagged proteins and peptides that can sense membrane organization.
We image sets of probes in intact cells under different biological or physical conditions. Here, cells are chemically fixed after the addition of a multivalent antigen against the B cell receptor, a condition that leads to immune signaling.
We then quantify the relative distribution of pairs of probes. In this case we find that an ordered phase marker partitions into BCR rich domains, while a disordered phase marker is excluded. Recruitment is temperature dependent, as expected for a lipid mediated process.